Course Summary

Learn the complete workflow of RNA-Seq — from essential biological principles and next-generation sequencing (NGS) concepts to practical data analysis. This no-coding course is designed for medical and biomedical learners who want to confidently perform RNA-Seq data analysis.

Course Description

This course introduces you to the world of NGS and transcriptomics in a clear, step-by-step manner. The focus is on building a broad conceptual understanding and hands-on analysis skills that empower you to perform RNA-Seq data analysis independently, without getting lost in complex statistics, technologies, or coding.

The course begins with the fundamental principles of gene expression and NGS, provides an overview of the RNA-Seq workflow, and then guides you through the complete RNA-Seq analysis pipeline using a user-friendly analysis environment that requires no coding skills.

Through detailed instructions with recorded practical videos in Bangla, live interactive sessions, curated external resources, readings, worksheets, and quizzes, you will learn to perform key steps in RNA-Seq data analysis, including quality control, read alignment, gene expression quantification, differential expression, and enrichment analysis. A real published dataset from an international peer-reviewed journal will be used throughout the course, allowing you to practice each step of the workflow in a structured, hands-on manner, and eventually reproduce the results of the published paper. In the final phase, you will complete a capstone project, presenting your findings in a format that simulates a research presentation, thus building your confidence and competence to independently perform RNA-Seq analyses.

By the end of the course, you will be able to:

• Explain key concepts and terminology in NGS and RNA-Seq.
• Perform RNA-Seq data analysis from raw reads to biological interpretation without any coding.
• Present your RNA-Seq analysis results in a format that mimics a research presentation.

This course is ideal for:

Undergraduate students, graduate students, postdocs, and professionals in medicine, biology, or related fields who have no prior coding or bioinformatics experience. It is particularly helpful for those who want to conduct research using RNA-Seq or prepare themselves for higher studies in competitive research programs.

Instructor:

This course will be taught by Dr. Md Anwarul Karim (Mijan), currently a Postdoctoral Researcher at Baylor College of Medicine, USA. Dr. Karim is a medical graduate from Chittagong Medical College and holds a PhD in Genetics from the University of Hong Kong.